Micropropagation of Rosmarinus Officinalis and Nigella Sativa for Internal Disease Applications
Keywords:
Micropropagation, Rosmarinus officinalis, Nigella sativa, internal diseases, bioprotectants, antioxidants, plant tissue culture, in vitro propagation, medicinal plants, hydroponic cultivation, biostimulants.Abstract
This article explores the micropropagation techniques of two highly valued medicinal plants: Rosmarinus officinalis (rosemary) and Nigella sativa (black seed). The study outlines protocols for in vitro cultivation using optimized plant tissue culture methods to produce disease-free and genetically stable plantlets. By utilizing Murashige and Skoog (MS) media with specific growth regulators, efficient shoot proliferation and root induction were achieved. Furthermore, the paper investigates the therapeutic applications of the bioactive compounds derived from these plants—such as rosmarinic acid, carnosic acid, and thymoquinone—in the prevention and treatment of various internal diseases, including inflammatory, oxidative stress-related, and microbial conditions. The integration of micropropagation with hydroponic systems is also discussed as a strategy to enhance the yield and quality of phytochemicals. Overall, the study supports the potential of these plants as natural alternatives or complementary agents in modern medicine and pharmaceutical industries.
References
Misra, P., & Chaturvedi, H. C. (1984). Micropropagation of Rosmarinus officinalis L. Plant Cell, Tissue and Organ Culture, 3, 163–168.
Vardanyan, A., Mairapetyan, S., Tadevosyan, A., Asatryan, A., Matevosyan, A., & Ghalachyan, L. (2021). Pharmacochemical investigation of Rosmarinus officinalis L. cultivated by clonal micropropagation and hydroponic combined method. Functional Foods in Health and Disease, 15(1), 1–15.
Villegas-Sánchez, E., Macías-Alonso, M., Osegueda-Robles, S., Herrera-Isidrón, L., Nuñez-Palenius, H., & González-Marrero, J. (2021). In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production. Pharmaceuticals, 14(8), 747.
Stamenov, D., et al. (2021). Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil. Contemporary Agriculture, 70(3-4), 108–115.