Micropropagation of Medicinal Plants: Techniques for Thyme (Thymus Vulgaris) and Rosemary (Rosmarinus Officinalis)
Keywords:
Micropropagation, Thymus vulgaris, Rosmarinus officinalis, tissue culture, plant growth regulators, acclimatization, genetic stability, essential oils.Abstract
Micropropagation is a vital biotechnological tool for the rapid and efficient multiplication of medicinal plants, ensuring the conservation of genetic traits and the production of high-quality plant material. This review focuses on the micropropagation protocols developed for Thymus vulgaris and Rosmarinus officinalis, two aromatic and medicinal species of significant economic importance. The paper discusses the selection of explants, the composition of culture media, the role of plant growth regulators, and the acclimatization processes, providing a comprehensive overview of the methodologies employed in the in vitro propagation of these plants.
References
El-Banna, H. A., & Abd El-Salam, M. A. (2018). Micropropagation of Thymus vulgaris L. through axillary shoot proliferation. Journal of Plant Production, 9(1), 79–86. https://jpp.journals.ekb.eg/article_41294.html
Makunga, N. P., & Staden, J. V. (2003). An efficient system for the production of clonal plantlets of Rosmarinus officinalis by shoot tip culture. Plant Cell, Tissue and Organ Culture, 74(2), 171–175. https://link.springer.com/article/10.1007/bf00033737
Sghir, F., Ait Ali, M., & Ennabili, A. (2011). In vitro regeneration of Thymus vulgaris L. using apical and nodal segments. In Vitro Cellular & Developmental Biology – Plant, 47, 751–757. https://link.springer.com/article/10.1007/s11627-011-9347-6
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Bajaj, Y. P. S. (Ed.). (1995). Biotechnology in Agriculture and Forestry: Medicinal and Aromatic Plants VIII. Springer-Verlag.
George, E. F., Hall, M. A., & De Klerk, G.-J. (2008). Plant Propagation by Tissue Culture: Volume 1. The Background. Springer.