Micropropagation of Medicinal Plants: Techniques for Thyme (Thymus Vulgaris) and Rosemary (Rosmarinus Officinalis)

Authors

  • Shokirova Gavkharkhan Nazigulomovna Senior Lecturer at the Department of "Botany, Biotechnology and Ecology", Fergana State University (PhD)
  • Rasulova Sarvinoz daughter of Khairulla Master's student

Keywords:

Micropropagation, Thymus vulgaris, Rosmarinus officinalis, tissue culture, plant growth regulators, acclimatization, genetic stability, essential oils.

Abstract

Micropropagation is a vital biotechnological tool for the rapid and efficient multiplication of medicinal plants, ensuring the conservation of genetic traits and the production of high-quality plant material. This review focuses on the micropropagation protocols developed for Thymus vulgaris and Rosmarinus officinalis, two aromatic and medicinal species of significant economic importance. The paper discusses the selection of explants, the composition of culture media, the role of plant growth regulators, and the acclimatization processes, providing a comprehensive overview of the methodologies employed in the in vitro propagation of these plants.

References

El-Banna, H. A., & Abd El-Salam, M. A. (2018). Micropropagation of Thymus vulgaris L. through axillary shoot proliferation. Journal of Plant Production, 9(1), 79–86. https://jpp.journals.ekb.eg/article_41294.html

Makunga, N. P., & Staden, J. V. (2003). An efficient system for the production of clonal plantlets of Rosmarinus officinalis by shoot tip culture. Plant Cell, Tissue and Organ Culture, 74(2), 171–175. https://link.springer.com/article/10.1007/bf00033737

Sghir, F., Ait Ali, M., & Ennabili, A. (2011). In vitro regeneration of Thymus vulgaris L. using apical and nodal segments. In Vitro Cellular & Developmental Biology – Plant, 47, 751–757. https://link.springer.com/article/10.1007/s11627-011-9347-6

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

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George, E. F., Hall, M. A., & De Klerk, G.-J. (2008). Plant Propagation by Tissue Culture: Volume 1. The Background. Springer.

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Published

2025-04-21

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Articles

How to Cite

Micropropagation of Medicinal Plants: Techniques for Thyme (Thymus Vulgaris) and Rosemary (Rosmarinus Officinalis). (2025). International Journal of Scientific Trends, 4(4), 69-72. https://scientifictrends.org/index.php/ijst/article/view/529